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Image Search Results
Journal: Cell host & microbe
Article Title: TRIM79?, an interferon-stimulated gene product, restricts tick-borne encephalitis virus replication by degrading the viral RNA polymerase
doi: 10.1016/j.chom.2011.08.004
Figure Lengend Snippet: (A) Western blot analysis of lysates from 293 cells transfected with TRIM79α-GFP or GFP plasmids and treated with CHX (100 μg/ml) for indicated times (h). TRIM79α-GFP blots were quantitated, normalized to β– actin, and presented as proportion of TRIM79α remaining over time. Error bars represent mean +/− SEM from three experiments. (B) Ubiquitination assay for Ub-modified TRIM79α. TRIM79α was affinity purified from lysates of 293 cells expressing TRIM79α-V5/AP, with or without HA-Ub or HA-SUMO1, and treated with DMSO or MG132 (10 μM) for 4 h. Conjugation of Ub or SUMO1 was visualized by probing blots with α-HA antibody. Western blot of input is shown to demonstrate expression levels of HA-Ub, SUMO1 and TRIM79α. M, mock transfected.
Article Snippet: Additional plasmids used were: pRK5-HA-ubiquitin (Ub)-WT and K0 (
Techniques: Western Blot, Transfection, Ubiquitin Assay, Modification, Affinity Purification, Expressing, Conjugation Assay